President Donald Trump’s tightening grip over the Justice Department to target his political opponents and lawmakers’ increasing calls for the release of more files from federal investigations into deceased sex offender Jeffrey Epstein are likely to take center stage at a contentious Senate hearing Tuesday for Attorney General Pam Bondi. 

The hearing before the Senate Judiciary Committee is the first time since July that Bondi has faced questions from lawmakers and follows a tumultuous summer for the department that included deployments of federal law enforcement to Democratic-run cities, a growing number of investigations announced into Trump’s political foes and the controversial indictment of former FBI Director James Comey. 

As ABC News first reported, the move to seek Comey’s indictment came over the objections of career prosecutors and followed Trump’s removal of his appointee to lead the U.S. Attorney’s Office for the Eastern District of Virginia, Erik Siebert, who expressed reservations about pursuing charges against Comey and New York Attorney General Letitia James, sources told ABC News.

Trump eventually installed a White House aide and former personal attorney Lindsey Halligan to lead the office and move forward with the case against Comey, and a grand jury narrowly voted to indict him on two counts of making false statements to Congress and obstructing a congressional investigation — while declining to indict on a third false statements charge. Comey has denied wrongdoing and is set to appear Thursday in federal court for his arraignment.

While sources told ABC News that leadership at DOJ expressed reservations about pursuing the case, Bondi and FBI Director Kash Patel went on to publicly cheer news of Comey’s indictment in news interviews and social media posts. 

The next week, the administration moved to fire a top national security prosecutor in the office, Michael Ben’Ary, over a misleading social media post that falsely suggested he was among the prosecutors who resisted charging Comey. 

Ben’Ary was leading a major case against one of the alleged plotters of the Abbey Gate bombing during the U.S. withdrawal from Afghanistan. In a scathing departure letter, Ben’Ary set his sights squarely on the Justice Department’s leadership and labeled his removal as just one in a series of recent moves taken to root out career officials for political reasons at the expense of the nation’s security. 

“This example highlights the most troubling aspect of the current operations of the Department of Justice: the leadership is more concerned with punishing the President’s perceived enemies than they are with protecting our national security,” Ben’Ary wrote. “Justice for Americans killed and injured by our enemies should not be contingent on what someone in the Department of Justice sees in their social media feed that day.”

The DOJ declined to comment when asked about Ben’Ary’s letter.

Those actions have caused unprecedented turmoil at the Eastern District, which oversees some of the nation’s most sensitive national security, terrorism and espionage investigations.

Current and former officials say that turmoil has reverberated further across the Justice Department’s workforce around the country, with attorneys concerned they’ll face professional repercussions if they resist taking part in politicized investigations or prosecutions.

On Monday, nearly 300 DOJ employees who left the department since Trump’s inauguration released a letter on the eve of Bondi’s hearing describing her leadership as “appalling” in its treatment of the career workforce and the elimination of longstanding norms of independence from the White House. 

“We call on Congress to exercise its oversight responsibilities far more vigorously,” the former employees said. “Members in both chambers and on both sides of the aisle must provide a meaningful check on the abuses we’re witnessing. And we call on all Americans — whose safety, prosperity, and rights depend on a strong DOJ — to speak out against its destruction.”

The DOJ declined to comment on the letter.

Bondi will likely also face heavy scrutiny over conflicting statements out of the administration on the Epstein files, after the Justice Department and FBI said in a July letter that no further releases were warranted and that there was no evidence suggesting others participated or enabled Epstein’s abuse of minor girls. 

Democrats have accused the administration of seeking to cover up any mentions of Trump or high-profile appointees who had past associations with Epstein, which the administration has denied. 

Trump and Epstein, who died by suicide in 2019 while awaiting trial on charges of trafficking young girls and women, were friends in the 1990s but the president said the relationship soured after Epstein poached some employees from Trump’s Florida club after he explicitly warned him not to do so.

An effort underway in the House of Representatives to hold a vote on a measure that would demand the administration release the entirety of the files has been put on hold after Speaker Mike Johnson sent the House home amid the ongoing government shutdown. 

The recent rise in acts of political violence will also likely be a central focus of questions to Bondi. Trump has recently ordered the department to ramp up investigations into so-called “radical left” organizations that he and other senior White House officials have alleged, without providing evidence, as helping to fund perpetrators who have attacked federal law enforcement officials dispatched around the country. 

Just days after Trump’s comments, a senior official in the Justice Department ordered several U.S. Attorney’s offices around the country to prepare to open sweeping criminal investigations in to the Open Society Foundations founded by billionaire George Soros, naming criminal statutes ranging from robbery, material support for terrorism and racketeering, ABC News previously confirmed.

In a statement, the Open Society Foundations called the accusations “politically motivated attacks on civil society, meant to silence speech the administration disagrees with and undermine the First Amendment right to free speech.”

In her most recent appearances before the House and Senate in late June, Bondi sought to brush off pointed questions from Democrats by repeatedly deflecting to crimes committed by undocumented immigrants in their states and districts that were among briefing materials she brought with her to the hearings. 

She has also dismissed any characterization of the Justice Department appearing to work in lockstep with the White House as “politicization” of law enforcement. Bondi and other senior DOJ officials have instead argued that the two federal cases brought against Trump by a special counsel under the Biden Administration represented a far more egregious example of weaponization, echoing grievances leveled at the department by Trump. 

“Whether you’re a former FBI director, whether you’re a former head of an intel community, whether you are current state and local elected official, whether you are a billionaire funding organizations to try to keep Donald Trump out of office — everything is on the table,” Bondi said in a Fox News appearance last month. “We will investigate and will end the weaponization — no longer will there be a two-tier system of justice.”